Effectiveness of Selected Materials Against Enterococcus faecalis: Part 3. The Antibacterial Effect of Calcium Hydroxide and Chlorhexidine on Enterococcus faecalis

It has been found that Enterococcus faecalis is most commonly isolated in failed endodontic treatment. Irrigation with chlorhexidine gluconate has been suggested based on its antimicrobial effect and substantivity. Calcium hydroxide also is an effective antimicrobial agent because of its high alkalinity. The purpose of this study was to test the individual and combined effect of calcium hydroxide and chlorhexidine against E. faecalis. The agar-diffusion test was performed on Mueller-Hinton plates. Paper disks were impregnated with: (a) CaOH powder with sterile water; (b) Pulpdent®; (c) 0.12% Peridex®; (d) CaOH powder with Peridex®; and (e) Pulpdent® with Peridex®. Ampicillin served as a control. The plates were incubated at 37°C for 72 h. Peridex® showed significantly larger zones of inhibition compared with CaOH. No statistically significant difference was found between Peridex® and the combination of CaOH and Peridex®.