Antisense DNA oligonucleotides I: The use of ionspray tandem mass spectrometry for the sequence verification of methylphosphonate oligodeoxyribonucleotides

The sequences of synthetically prepared methylphosphonate oligodeoxyribonucleotides have been verified using ionspray tandem mass spectrometry with sample introduction via flow injection. The technique involves the use of product‐ion scans from multiply protonated (4+ and 5+) precursors. Among the i...

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Veröffentlicht in:	Rapid communications in mass spectrometry 1993-03, Vol.7 (3), p.190-194
Hauptverfasser:	Baker, Timothy R., Keough, Thomas, Dobson, Roy L. M., Riley, Timothy A., Hasselfield, Jody A., Hesselberth, P. Eric
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Chemistry Exact sciences and technology Mass Spectrometry Molecular Sequence Data Oligonucleotides, Antisense - chemistry Organic chemistry Organophosphonates - chemistry Reactivity and mechanisms
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Beschreibung
Zusammenfassung:	The sequences of synthetically prepared methylphosphonate oligodeoxyribonucleotides have been verified using ionspray tandem mass spectrometry with sample introduction via flow injection. The technique involves the use of product‐ion scans from multiply protonated (4+ and 5+) precursors. Among the ions detected are several series of fragments of different charge states that indicate the base sequence of the intact molecule. Oligomers as large as 18 bases have been successfully characterized.
ISSN:	0951-4198 1097-0231
DOI:	10.1002/rcm.1290070305