Morphological analysis of Yarrowia lipolytica under stress conditions through image processing

Stress conditions (thermal and oxidative) were applied to Yarrowia lipolytica culture. A rearrangement in cell metabolism as well as dimorphism was observed under these conditions. An image analysis procedure was employed for morphology characterization, and a net increase of around 25% on hyphae fo...

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Veröffentlicht in:Bioprocess and biosystems engineering 2003-07, Vol.25 (6), p.371-375
Hauptverfasser: Kawasse, F M, Amaral, P F, Rocha-Leão, M H M, Amaral, A L, Ferreira, E C, Coelho, M A Z
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Sprache:eng
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Zusammenfassung:Stress conditions (thermal and oxidative) were applied to Yarrowia lipolytica culture. A rearrangement in cell metabolism as well as dimorphism was observed under these conditions. An image analysis procedure was employed for morphology characterization, and a net increase of around 25% on hyphae formation was detected. A significant increment in total hyphal length was detected, compared with the control system. The results obtained lead to the consideration of a possible relationship between dimorphism and a cell response mechanism to stress conditions.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-003-0319-z