A New Method for the Preparation of Acyl-CoA Thioesters

A New Method for the Preparation of Acyl-CoA Thioesters

A method is described for the preparation of CoA thioesters of fatty acids. Acyl-CoA thioester was synthesized by way of 1-acylimidazole and purified by high performance liquid chromatography. This method is applicable to the preparation of a variety of acyl-CoA thioesters of various chain lengths (...

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Veröffentlicht in:Journal of biochemistry (Tokyo) 1981-01, Vol.89 (2), p.337-339
Hauptverfasser: KAWAGUCHI, Akihiko, YOSHlMURA, Tsutomu, OKUDA, Shigenobu
Format: Artikel
Sprache:eng
Schlagworte:
Acyl Coenzyme A - chemical synthesis
Chromatography, High Pressure Liquid
Coenzyme A
Imidazoles
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Zusammenfassung:A method is described for the preparation of CoA thioesters of fatty acids. Acyl-CoA thioester was synthesized by way of 1-acylimidazole and purified by high performance liquid chromatography. This method is applicable to the preparation of a variety of acyl-CoA thioesters of various chain lengths (from C8 to C18) and with varying degrees of unsaturation. It is particularly suitable for preparing acyl-CoA thioesters of labeled fatty acids or fatty acids available only in small amounts.
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a133207