Growth factor modulation of surface ganglioside expression in cloned neoplastic glia

Growth factor modulation of surface ganglioside expression in cloned neoplastic glia

Cloned neoplastic astrocytes from a human glioma-derived cell line (IPSB-18) were grown in fetal calf serum (FCS)-supplemented culture medium in the presence of three growth factors. Basic fibroblast growth factor (bFGF), epidermal growth factor (EGF) but not platelet-derived growth factor (PDGF) in...

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Veröffentlicht in:Neuroscience letters 1993-01, Vol.149 (1), p.1-5
Hauptverfasser: Pilkington, Geoffrey J., Dunan, Julie R., Rogers, Joan P., Clarke, Tracey M., Knott, Johanna C.A.
Format: Artikel
Sprache:eng
Schlagworte:
Astrocytes - drug effects
Astrocytes - metabolism
Biological and medical sciences
Cell coat. Cell surface
Cell structures and functions
Epidermal Growth Factor - pharmacology
Fibroblast Growth Factor 2 - pharmacology
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Ganglioside
Gangliosides - metabolism
Glia
Glioma - metabolism
Glioma - pathology
Growth factor
Histocytochemistry
Humans
Molecular and cellular biology
Neoplasm
Platelet-Derived Growth Factor - pharmacology
Tumor Cells, Cultured
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Zusammenfassung:Cloned neoplastic astrocytes from a human glioma-derived cell line (IPSB-18) were grown in fetal calf serum (FCS)-supplemented culture medium in the presence of three growth factors. Basic fibroblast growth factor (bFGF), epidermal growth factor (EGF) but not platelet-derived growth factor (PDGF) induced an increase in the number of cells positive for the ganglioside-recognizing monoclonal antibody, A2B5. No such growth factor-mediated induction could be detected in cells maintained in plasma-derived serum (PDS)-supplemented medium. Small molecules, removed from PDS during dialysis, may, therefore, act synergistically with growth factors in the control of ganglioside synthesis.
ISSN:0304-3940
1872-7972
DOI:10.1016/0304-3940(93)90332-F