Analysis of Apolipoprotein Size Distribution by Electroimmunoblotting from Non-Denaturing Composite Gels

An exponential gradient gel with 0–35% acrylamide and 0·5% agarose was developed for electrophoresis of intact lipoproteins with subsequent electroimmunoblotting. The system resolved in a single gel lipoprotein-associated proteins of sizes from ‘free’ apoproteins to VLDL. Reproducibility between gel...

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Veröffentlicht in:	Annals of clinical biochemistry 1993-03, Vol.30 (2), p.157-162
Hauptverfasser:	Lee, Lucille T, Maldonado, Blanca R, Roheim, Paul S, Thompson, James J
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Apolipoproteins - blood Apolipoproteins - isolation & purification Biological and medical sciences Electrophoresis, Agar Gel Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Humans Hyperlipidemia, Familial Combined - blood Immunoblotting - methods Lipoproteins, HDL - blood Lipoproteins, HDL - isolation & purification Lipoproteins, LDL - blood Lipoproteins, LDL - isolation & purification Lipoproteins, myelin Lipoproteins, VLDL - blood Lipoproteins, VLDL - isolation & purification Proteins
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Beschreibung
Zusammenfassung:	An exponential gradient gel with 0–35% acrylamide and 0·5% agarose was developed for electrophoresis of intact lipoproteins with subsequent electroimmunoblotting. The system resolved in a single gel lipoprotein-associated proteins of sizes from ‘free’ apoproteins to VLDL. Reproducibility between gels was good (coefficient of variation
ISSN:	0004-5632 1758-1001
DOI:	10.1177/000456329303000208