Studies on the secondary structure of 5.8 S rRNA from a thermophile [fungus], Thermomyces lanuginosus
The nucleotide sequence of ribosomal 5.8 S RNA from a thermophilic fungus, Thermomyces lanuginosus, was determined to be (formula: see text). The secondary structure was probed under a variety of ionic conditions using limited pancreatic and T1 ribonuclease digestion and rapid gel sequencing techniq...
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Veröffentlicht in: | The Journal of biological chemistry 1981-06, Vol.256 (11), p.5675-5682 |
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Sprache: | eng |
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Zusammenfassung: | The nucleotide sequence of ribosomal 5.8 S RNA from a thermophilic fungus, Thermomyces lanuginosus, was determined to be (formula:
see text). The secondary structure was probed under a variety of ionic conditions using limited pancreatic and T1 ribonuclease
digestion and rapid gel sequencing techniques. The results generally supported the "burp gun" model previously proposed for
all 5.8 S rRNAs (Nazar, R. N., Sitz, T. O., and Busch, H. (1975) J. Biol. Chem. 250, 8591-8597) and were inconsistent with
a recently suggested "cloverleaf" configuration (Luoma, G. A., and Marshall, A. G. (1978) Proc. Natl. Acad. Sci. U.S.A. 75,
4901-4905). Theoretical considerations of the overall stability also appear to favor the former estimate. As previously observed
with other 5.8 S RNAs the sequence of T. lanuginosus RNA is strikingly homologous with other species; it differs in only 13
positions from that of yeast. When compared to yeast, the differences appear not to contribute to the stability of the secondary
structure but probably lead to a more stable 5.8 S-25 S rRNA interaction in the large ribosomal subunit. A general comparison
of T. lanuginosus 5.8 S RNA with all known 5.8 S RNA sequences indicates that, although modified nucleotides differ significantly
between species, they are always located in four highly conserved regions of the 5.8 S molecule, probably contributing to
the unique character of these very essential sequences. |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/S0021-9258(19)69258-6 |