Crystals of β-Xylanase from Aspergillus oryzae

An endo-xylanase was isolated from the culture of fungus Aspergillus oryzae variant D5. The purified enzyme had a molecular weight of 24,000 and the isoelectric point of 3·6. Xylanase crystals were obtained from a polyethylene glycol 6000 solution by the hanging-drop method. Seeding was used for the...

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Veröffentlicht in:	Journal of molecular biology 1993-03, Vol.230 (2), p.661-663
Hauptverfasser:	Golubev, A.M., Kilimnik, A.Yu, Neustroev, K.N., Pickersgill, R.W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aspergillus oryzae Aspergillus oryzae - enzymology Crystallization crystals Endo-1,4-beta Xylanases fungus Glycoside Hydrolases - chemistry Glycoside Hydrolases - isolation & purification Glycoside Hydrolases - metabolism Kinetics Polyethylene Glycols xylanase
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Beschreibung
Zusammenfassung:	An endo-xylanase was isolated from the culture of fungus Aspergillus oryzae variant D5. The purified enzyme had a molecular weight of 24,000 and the isoelectric point of 3·6. Xylanase crystals were obtained from a polyethylene glycol 6000 solution by the hanging-drop method. Seeding was used for the enlargement of the crystal size. Crystals belong to the monoclinic space group P21 with cell dimensions a = 54·9 Å, b = 74·5 Å, c = 50·8 Å, and β = 108·7°. Crystals diffract beyond 2·5 Å resolution.
ISSN:	0022-2836 1089-8638
DOI:	10.1006/jmbi.1993.1177