Typing of murine cell-surface antigens by cellular radioimmunoassay

Typing of murine cell-surface antigens by cellular radioimmunoassay

A cellular radioimmunoassay utilizing 125I-labelled Protein A was used for detecting antigen-antibody complexes on glutaraldehyde fixed cells attached to microtiter plates. This method is rapid, sensitive and specific for revealing H-2 private and public specificities as well as Ia and Lyt antigens....

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Veröffentlicht in:Journal of immunological methods 1981-01, Vol.41 (3), p.333-341
Hauptverfasser: Levy, D., Hoang-Xuan, M., Colombani, M.J., Zilber, M.Th, Leclerc, J.C., Levy, J.P.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Antigen-Antibody Reactions
Antigens, Surface - analysis
Cytotoxicity, Immunologic
H-2 Antigens - analysis
Histocompatibility Antigens Class II - analysis
Lymphocytes - immunology
Mice
Radioimmunoassay - methods
Staphylococcal Protein A - immunology
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Beschreibung
Zusammenfassung:A cellular radioimmunoassay utilizing 125I-labelled Protein A was used for detecting antigen-antibody complexes on glutaraldehyde fixed cells attached to microtiter plates. This method is rapid, sensitive and specific for revealing H-2 private and public specificities as well as Ia and Lyt antigens. As plates may be kept for months, several reactivities can be tested in one step on a large panel rendering a regular supply of animals unnecessary.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(81)90195-2