Isolating RNA from whole blood — the dawn of RNA-based diagnosis?

A novel cationic surfactant solution is used to lyse blood cells and precipitate RNA and DNA. The RNA is recovered by extracting the pellet with a small volume of guanidinium isothiocyanate or formamide.

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nature (London) 1993-03, Vol.362 (6416), p.186-188
Hauptverfasser:	Macfarlane, Donald E., Dahle, Christopher E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Blood Deoxyribonucleic acid Diverse techniques DNA DNA - blood DNA - isolation & purification Fundamental and applied biological sciences. Psychology Genes, abl Humanities and Social Sciences Humans Indicators and Reagents Leukemia, Myelogenous, Chronic, BCR-ABL Positive Molecular and cellular biology Molecular Sequence Data multidisciplinary Oligodeoxyribonucleotides Oncogenes Polymerase Chain Reaction - methods product-review Ribonucleic acid RNA RNA - blood RNA - genetics RNA - isolation & purification RNA-Directed DNA Polymerase Science Science (multidisciplinary) Surface-Active Agents Surfactants Tumor Cells, Cultured
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A novel cationic surfactant solution is used to lyse blood cells and precipitate RNA and DNA. The RNA is recovered by extracting the pellet with a small volume of guanidinium isothiocyanate or formamide.
ISSN:	0028-0836 1476-4687
DOI:	10.1038/362186a0