Lethal heat induces single strand breaks in the DNA of bacterial spores

Lethal heat induces single strand breaks in the DNA of bacterial spores

Lethal heating induces DNA single strand breakage in bacterial endospores as detected by the alkaline sucrose gradient centrifugation technique. Heating of spores of Bacillus subtilis 168 at 90°C for 10, 30, and 60 min induced 6, 15, and 15 single strand breaks, respectively and inactivated 6%, 98.2...

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Veröffentlicht in:Biochemical and biophysical research communications 1981-01, Vol.98 (1), p.191-196
Hauptverfasser: Grecz, Nicholas, Bruszer, George
Format: Artikel
Sprache:eng
Schlagworte:
Bacillus subtilis - metabolism
DNA, Bacterial - metabolism
DNA, Single-Stranded - metabolism
Hot Temperature
Kinetics
Spores, Bacterial - metabolism
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Zusammenfassung:Lethal heating induces DNA single strand breakage in bacterial endospores as detected by the alkaline sucrose gradient centrifugation technique. Heating of spores of Bacillus subtilis 168 at 90°C for 10, 30, and 60 min induced 6, 15, and 15 single strand breaks, respectively and inactivated 6%, 98.2%, and 99.974% of the spores. This is the first report to our knowledge identifying specifically single strand DNA breakage with lethal heat injury of bacterial spores.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(81)91887-8