Detection of the amine derivative of misonidazole in human urine by high-pressure liquid chromatography

Detection of the amine derivative of misonidazole in human urine by high-pressure liquid chromatography

A sensitive high-pressure liquid chromatographic assay for the detection and quantitation of the amine derivative of misonidazole in human urine is reported. The amine was converted to its dansyl derivative and was separated on a reverse-phase μBondapak phenyl column. Absorbance at 365 nm was monito...

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Veröffentlicht in:Analytical biochemistry 1981, Vol.110 (1), p.197-200
1. Verfasser: Varghese, A.J.
Format: Artikel
Sprache:eng
Schlagworte:
Chromatography, High Pressure Liquid
Dansyl Compounds - urine
Female
Humans
Misonidazole - urine
Nitroimidazoles - urine
Spectrophotometry, Ultraviolet
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Zusammenfassung:A sensitive high-pressure liquid chromatographic assay for the detection and quantitation of the amine derivative of misonidazole in human urine is reported. The amine was converted to its dansyl derivative and was separated on a reverse-phase μBondapak phenyl column. Absorbance at 365 nm was monitored for the detection of the dansyl derivative. Using this procedure, the amine derivative has been identified as a urinary metabolite of misonidazole.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(81)90135-4