Nitrogen-13--Labeled Nitrite and Nitrate: Distribution and Metabolism after Intratracheal Administration

Radioactive nitrogen-13 from nitrite (NO$_{2}{}^{-}$) or nitrate (NO$_{3}{}^{-}$) administered intratracheally or intravenously without added carrier to mice or rabbits was distributed evenly throughout most organs and tissues regardless of the entry route or the anion administered. Nitrogen-13 from...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Science (American Association for the Advancement of Science) 1981-04, Vol.212 (4490), p.58-61
Hauptverfasser: Parks, Norris J., Krohn, Kenneth A., Mathis, Chester A., Chasko, Joseph H., Geiger, Kenneth R., Gregor, Marsha E., Peek, Neal F.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Radioactive nitrogen-13 from nitrite (NO$_{2}{}^{-}$) or nitrate (NO$_{3}{}^{-}$) administered intratracheally or intravenously without added carrier to mice or rabbits was distributed evenly throughout most organs and tissues regardless of the entry route or the anion administered. Nitrogen-13 from both anions was distributed uniformly between plasma and blood cells. We found rapid in vivo oxidation of NO$_{2}{}^{-}$ to NO$_{3}{}^{-}$ at concentrations of 2 to 3 nanomoles per liter in blood. Over 50 percent oxidation within 10 minutes accounted for the similar nitrogen-13 distributions from both parent ions. The oxidation rates were animal species--dependent. No reduction of $^{13}$NO$_{3}{}^{-}$ to $^{13}$NO$_{2}{}^{-}$ was observed. A mechanistic hypothesis invoking oxidation of $^{13}$NO$_{2}{}^{-}$ by a catalase--hydrogen peroxide complex accounts for the results. These results imply a concentration dependence for the in vivo fate of NO$_{2}{}^{-}$ or nitrogen dioxide.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.7209517