Identification of hemoglobin derivatives by means of electrofocusing

Identification of hemoglobin derivatives by means of electrofocusing

A method for the identification and quantitation of hemoglobin derivatives is described. Samples diluted 1 in 100 were applied to precoated polyacrylamide-gel plates containing an Ampholine gradient of pH 3.5 – 9.5 and focused for 1.5 h at a constant 40 W. All hemoglobins under investigation were ch...

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Veröffentlicht in:Forensic science international 1981, Vol.17 (1), p.45-49
Hauptverfasser: Bonte, W., Jursch, R., Straube, J.
Format: Artikel
Sprache:eng
Schlagworte:
cardiovascular system
electrofocusing
Hemoglobins - analysis
Humans
Isoelectric Focusing
monitoring
monitoring measurements
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Zusammenfassung:A method for the identification and quantitation of hemoglobin derivatives is described. Samples diluted 1 in 100 were applied to precoated polyacrylamide-gel plates containing an Ampholine gradient of pH 3.5 – 9.5 and focused for 1.5 h at a constant 40 W. All hemoglobins under investigation were characterized by specific patterns which allow them to be differentiated. Electrofocusing is recommended for screening tests, but quantitative evaluation using densitometry is possible as well.
ISSN:0379-0738
1872-6283
DOI:10.1016/0379-0738(81)90188-2