Potassium-stimulated, calcium-dependent release of [ 3H]GABA from neuron- and glia-enriched cultures of cells dissociated from rat cerebellum

The release of [ 3H]GABA from cultures of cells dissociated from the rat cerebellum was investigated. The culture system contained a population of neurons (stellate and basket cells) which were capable of accumulating [ 3H]GABA and releasing it in a calcium-dependent manner in response to 50 mM pota...

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Veröffentlicht in:	Brain research 1981-02, Vol.206 (2), p.485-489
Hauptverfasser:	Pearce, Brian R., Neil Currie, D., Beale, Richard, Dutton, Gary R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Calcium - pharmacology cell cultures cerebellum Cerebellum - drug effects Cerebellum - metabolism Culture Techniques dissociated cells GABA gamma-Aminobutyric Acid - metabolism Neuroglia - drug effects Neurons - drug effects Potassium - pharmacology Rats Synaptic Transmission - drug effects transmitter release
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Zusammenfassung:	The release of [ 3H]GABA from cultures of cells dissociated from the rat cerebellum was investigated. The culture system contained a population of neurons (stellate and basket cells) which were capable of accumulating [ 3H]GABA and releasing it in a calcium-dependent manner in response to 50 mM potassium. In addition, the release of [ 3H]GABA for cultured astrocytes was found to be insensitive to potassium depolarization.
ISSN:	0006-8993 1872-6240
DOI:	10.1016/0006-8993(81)90552-7