λSHK and λAASV: phage vectors for efficient cDNA cloning and expression in mammalian cells
We describe here the construction of two hybrid λ phage vectors for the preparation of directional cDNA libraries that can be used directly, or after their conversion to plasmids, for expression in mammalian cells.
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| Veröffentlicht in: | Gene 1993-01, Vol.123 (2), p.287-288 |
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| container_end_page | 288 |
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| container_issue | 2 |
| container_start_page | 287 |
| container_title | Gene |
| container_volume | 123 |
| creator | Swaroop, Anand Ganguly, Subinay Sarkar, Saumyendra N. Vasavada, Haren A. |
| description | We describe here the construction of two hybrid λ phage vectors for the preparation of directional cDNA libraries that can be used directly, or after their conversion to plasmids, for expression in mammalian cells. |
| doi_str_mv | 10.1016/0378-1119(93)90140-X |
| format | Article |
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Psychology ; Gene Library ; Genetic engineering ; Genetic technics ; Genetic Vectors - genetics ; libraries ; Mammals ; Methods. Procedures. Technologies ; phage lambda ; Recombinant DNA ; Transfection ; Vectors (cloning, transfer, expression). 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Psychology</subject><subject>Gene Library</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Vectors - genetics</subject><subject>libraries</subject><subject>Mammals</subject><subject>Methods. Procedures. Technologies</subject><subject>phage lambda</subject><subject>Recombinant DNA</subject><subject>Transfection</subject><subject>Vectors (cloning, transfer, expression). 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| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1993-01, Vol.123 (2), p.287-288 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75580515 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Bacteriophage lambda - genetics bacteriophage λ Biological and medical sciences Biotechnology Cloning, Molecular - methods Deoxyribonucleases, Type II Site-Specific DNA Replication - genetics Escherichia coli Fundamental and applied biological sciences. Psychology Gene Library Genetic engineering Genetic technics Genetic Vectors - genetics libraries Mammals Methods. Procedures. Technologies phage lambda Recombinant DNA Transfection Vectors (cloning, transfer, expression). Insertion sequences and transposons |
| title | λSHK and λAASV: phage vectors for efficient cDNA cloning and expression in mammalian cells |
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