λSHK and λAASV: phage vectors for efficient cDNA cloning and expression in mammalian cells

We describe here the construction of two hybrid λ phage vectors for the preparation of directional cDNA libraries that can be used directly, or after their conversion to plasmids, for expression in mammalian cells.

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Bibliographische Detailangaben
Veröffentlicht in:	Gene 1993-01, Vol.123 (2), p.287-288
Hauptverfasser:	Swaroop, Anand, Ganguly, Subinay, Sarkar, Saumyendra N., Vasavada, Haren A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteriophage lambda - genetics bacteriophage λ Biological and medical sciences Biotechnology Cloning, Molecular - methods Deoxyribonucleases, Type II Site-Specific DNA Replication - genetics Escherichia coli Fundamental and applied biological sciences. Psychology Gene Library Genetic engineering Genetic technics Genetic Vectors - genetics libraries Mammals Methods. Procedures. Technologies phage lambda Recombinant DNA Transfection Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Beschreibung
Zusammenfassung:	We describe here the construction of two hybrid λ phage vectors for the preparation of directional cDNA libraries that can be used directly, or after their conversion to plasmids, for expression in mammalian cells.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(93)90140-X