In situ hypothermic renal perfusion Evaluation of three intracellular solutions

Description of a simple technique for in situ hypothermic renal perfusion is presented. Three intracellular solutions simulating perfusates were tried on three groups of mongrel dogs with a fourth group that served as control. Right nephrectomy was performed on all dogs. The left kidney was perfused for two and one-half hours at a temperature of 15° to 17° C. Follow-up evaluation by estimation of five enzymes, glutamic oxaloacetic transaminase, alkaline phosphatase, lactic dehydrogenase, sorbitol dehydrogenase, and phospholexose isomerase in serum, urine, and renal tissue was conducted. Renal function was evaluated by the level of serum creatinine and its twenty-four-hour urinary excretion. Minimal biochemical changes comparable to the control group were observed in the group perfused by Chapman solution.