Organization of a 117-kb circular mitochondrial chromosome in IR36 rice

The organization of the mitochondrial genome in the IR36 indica variety of rice was analyzed using constant-field and CHEF gel electrophoresis. The minimum complexity of the genome is estimated to be 300 kb. There was no evidence for a master circular molecule of this size. The genome exists in vivo in multiple subgenomic circular molecules or mitochondrial chromosomes. Using several rare-cutting restriction enzymes a restriction map was constructed for a 117-kb circular chromosome. 6 genetic loci have been placed on this chromosome; cox1 and atp1 have been assigned precise positions while 4 additional genes (rrn26, cob2, atp6 and rrn18) were mapped to restriction fragments.