One-step enrichment of nucleated red blood cells: A potential application in perinatal diagnosis

We describe a discontinuous triple density gradient to obtain a 25-fold enrichment of nucleated red blood cells from mononuclear cells, granulocytes, and mature red blood cells in a single centrifugation step.

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Bibliographische Detailangaben
Veröffentlicht in:	Journal of immunological methods 1993-02, Vol.158 (2), p.277-280
Hauptverfasser:	Bhat, Neelima M., Bieber, Marcia M., Teng, Nelson N.H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, CD Antigens, Differentiation, B-Lymphocyte Antigens, Differentiation, Myelomonocytic Biological and medical sciences CD13 Antigens CD71 Cell Isolation Cell Separation - methods Centrifugation, Density Gradient - methods Diverse techniques Erythrocytes - immunology Female Fetal Blood - cytology Flow Cytometry Fundamental and applied biological sciences. Psychology Humans Infant, Newborn Infant, Newborn, Diseases - diagnosis Infant, Newborn, Diseases - genetics Leukocyte Common Antigens Molecular and cellular biology Nucleated red blood cell Pregnancy - blood Receptors, Transferrin
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Beschreibung
Zusammenfassung:	We describe a discontinuous triple density gradient to obtain a 25-fold enrichment of nucleated red blood cells from mononuclear cells, granulocytes, and mature red blood cells in a single centrifugation step.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(93)90224-U