Mechanism of luteolysis: Effect of estradiol and prostaglandin F2α on corpus luteum luteinizing hormone/human chorionic gonadotropin receptors and cyclic nucleotides in the rhesus monkey

Recent studies from our laboratory suggest that estrogen-induced luteolysis in the primate may be mediated through intraovarian synthesis of prostaglandin F2α (PGF2α). To elucidate further the mechanism of luteolysis, normally cycling rhesus monkeys received intra-corpus luteum (CL) injections of estradiol (100 μg), PGF2α (500 μg), or the appropriate vehicles on the seventh day after the preovulatory estradiol surge. Peripheral vein blood was drawn at 30-minute intervals for 6 hours for progesterone and luteinizing hormone (LH) assays. Five hours after intra-CL injection, the CL was excised and the LH/human chorionic gonadotropin (hCG) receptor-binding activity and cyclic guanosine monophosphate (cGMP) and cyclic adenosine monophosphate (cAMP) were measured. PGF2α significantly (p < 0.05) lowered progesterone within 1 hour, while the time required for estradiol to lower progesterone significantly was 3.5 hours; there was no significant change in LH. Estradiol and PGF2α significantly (p < 0.05) decreased the LH receptor-binding capacity at 5 hours, without any change in the binding affinity. Also, PGF2α significantly (p < 0.05) increased cGMP in the CL, while cAMP remained unchanged; estradiol treatment resulted in a significant (p < 0.05) increase in cAMP with no change in cGMP. This study suggests that estradiol and PGF2α cause a decrease in progesterone secretion by a loss of the LH/hCG receptor and the PGF2α may act further through the cGMP system.