A method for culturing human hair follicle cells
SUMMARY For the first time a method for culturing human hair follicle cells is described. The bovine eye lens capsule, a basement membrane‐like structure, is used as the substrate for the cultures. In a culture medium supplemented with hydrocortisone and insulin about 70% of the original follicles w...
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| Veröffentlicht in: | British journal of dermatology (1951) 1981-01, Vol.104 (1), p.1-5 |
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| container_title | British journal of dermatology (1951) |
| container_volume | 104 |
| creator | Weterings, P.J.J.M. Vermorken, A.J.M. Bloemendal†, H. |
| description | SUMMARY
For the first time a method for culturing human hair follicle cells is described. The bovine eye lens capsule, a basement membrane‐like structure, is used as the substrate for the cultures. In a culture medium supplemented with hydrocortisone and insulin about 70% of the original follicles will form growing colonies of diploid keratinocytes. |
| doi_str_mv | 10.1111/j.1365-2133.1981.tb01704.x |
| format | Article |
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For the first time a method for culturing human hair follicle cells is described. The bovine eye lens capsule, a basement membrane‐like structure, is used as the substrate for the cultures. In a culture medium supplemented with hydrocortisone and insulin about 70% of the original follicles will form growing colonies of diploid keratinocytes.</description><identifier>ISSN: 0007-0963</identifier><identifier>EISSN: 1365-2133</identifier><identifier>DOI: 10.1111/j.1365-2133.1981.tb01704.x</identifier><identifier>PMID: 7006669</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Cell Count ; Cells, Cultured ; Cytological Techniques - instrumentation ; Hair - cytology ; Hair - drug effects ; Humans ; Hydrocortisone - pharmacology ; Insulin - pharmacology</subject><ispartof>British journal of dermatology (1951), 1981-01, Vol.104 (1), p.1-5</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4051-dcf5304508df31c6f2499295b447d26018d2600b43507dfeb0ff3c1fad86715f3</citedby><cites>FETCH-LOGICAL-c4051-dcf5304508df31c6f2499295b447d26018d2600b43507dfeb0ff3c1fad86715f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2133.1981.tb01704.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2133.1981.tb01704.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7006669$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weterings, P.J.J.M.</creatorcontrib><creatorcontrib>Vermorken, A.J.M.</creatorcontrib><creatorcontrib>Bloemendal†, H.</creatorcontrib><title>A method for culturing human hair follicle cells</title><title>British journal of dermatology (1951)</title><addtitle>Br J Dermatol</addtitle><description>SUMMARY
For the first time a method for culturing human hair follicle cells is described. The bovine eye lens capsule, a basement membrane‐like structure, is used as the substrate for the cultures. In a culture medium supplemented with hydrocortisone and insulin about 70% of the original follicles will form growing colonies of diploid keratinocytes.</description><subject>Cell Count</subject><subject>Cells, Cultured</subject><subject>Cytological Techniques - instrumentation</subject><subject>Hair - cytology</subject><subject>Hair - drug effects</subject><subject>Humans</subject><subject>Hydrocortisone - pharmacology</subject><subject>Insulin - pharmacology</subject><issn>0007-0963</issn><issn>1365-2133</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkD1PwzAQhi0EKqXwE5AiBraEcxzbCWKBAoVSYOCjo5UPm6Y4TbET0f57EiXqzg2-4b3nznoQOsPg4aYulh4mjLo-JsTDUYi9KgHMIfA2e2i4i_bREAC4CxEjh-jI2iUAJkBhgAYcgDEWDRFcO4WsFmXmqNI4aa2r2uSrL2dRF_HKWcS5aQKt81RLJ5Va22N0oGJt5UnfR-jj_u59_ODOXieP4-uZmwZAsZulihIIKISZIjhlyg-iyI9oEgQ88xngsH0hCQgFnimZgFIkxSrOQsYxVWSEzru9a1P-1NJWosht-4N4JcvaCk4pDikJmsHLbjA1pbVGKrE2eRGbrcAgWl1iKVononUiWl2i1yU2DXzaX6mTQmY7tPfT5Fdd_ptruf3HZnEzvcUN7nZ4biu52eGx-RaME07F_GUiPqdP_twnb-KZ_AGfN4cL</recordid><startdate>198101</startdate><enddate>198101</enddate><creator>Weterings, P.J.J.M.</creator><creator>Vermorken, A.J.M.</creator><creator>Bloemendal†, H.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198101</creationdate><title>A method for culturing human hair follicle cells</title><author>Weterings, P.J.J.M. ; Vermorken, A.J.M. ; Bloemendal†, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4051-dcf5304508df31c6f2499295b447d26018d2600b43507dfeb0ff3c1fad86715f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Cell Count</topic><topic>Cells, Cultured</topic><topic>Cytological Techniques - instrumentation</topic><topic>Hair - cytology</topic><topic>Hair - drug effects</topic><topic>Humans</topic><topic>Hydrocortisone - pharmacology</topic><topic>Insulin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weterings, P.J.J.M.</creatorcontrib><creatorcontrib>Vermorken, A.J.M.</creatorcontrib><creatorcontrib>Bloemendal†, H.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of dermatology (1951)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weterings, P.J.J.M.</au><au>Vermorken, A.J.M.</au><au>Bloemendal†, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A method for culturing human hair follicle cells</atitle><jtitle>British journal of dermatology (1951)</jtitle><addtitle>Br J Dermatol</addtitle><date>1981-01</date><risdate>1981</risdate><volume>104</volume><issue>1</issue><spage>1</spage><epage>5</epage><pages>1-5</pages><issn>0007-0963</issn><eissn>1365-2133</eissn><abstract>SUMMARY
For the first time a method for culturing human hair follicle cells is described. The bovine eye lens capsule, a basement membrane‐like structure, is used as the substrate for the cultures. In a culture medium supplemented with hydrocortisone and insulin about 70% of the original follicles will form growing colonies of diploid keratinocytes.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>7006669</pmid><doi>10.1111/j.1365-2133.1981.tb01704.x</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-0963 |
| ispartof | British journal of dermatology (1951), 1981-01, Vol.104 (1), p.1-5 |
| issn | 0007-0963 1365-2133 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75518534 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Cell Count Cells, Cultured Cytological Techniques - instrumentation Hair - cytology Hair - drug effects Humans Hydrocortisone - pharmacology Insulin - pharmacology |
| title | A method for culturing human hair follicle cells |
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