RNA inhibits estrogen receptor binding to DNA

Cytosol from MTW9 rat mammary tumor contains a high molecular weight inhibitor of estrogen receptor binding to DNA. RNase treatment of crude preparations destroys inhibitory activity, whereas DNase or trypsin treatment are without affect, suggesting that some RNA molecule might be involved; in addit...

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Veröffentlicht in:	The Journal of biological chemistry 1981-02, Vol.256 (3), p.1145-1148
Hauptverfasser:	Feldman, M, Kallos, J, Hollander, V P
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Line Chromatography, Affinity Cytosol - metabolism Deoxyribonucleases DNA - metabolism Female Kinetics Mammary Neoplasms, Experimental - metabolism Polyribonucleotides - pharmacology Protein Binding Rats Receptors, Estrogen - isolation & purification Receptors, Estrogen - metabolism Ribonucleases RNA, Neoplasm - metabolism Trypsin
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Beschreibung
Zusammenfassung:	Cytosol from MTW9 rat mammary tumor contains a high molecular weight inhibitor of estrogen receptor binding to DNA. RNase treatment of crude preparations destroys inhibitory activity, whereas DNase or trypsin treatment are without affect, suggesting that some RNA molecule might be involved; in addition, RNA extracted from tumor cytosol was an effective inhibitor of estrogen receptor binding to DNA. A number of synthetic and natural RNA species were analyzed for inhibitory activity; a definite specificity was observed, with poly(G) > poly(U) > poly(A) or poly(C).
ISSN:	0021-9258 1083-351X
DOI:	10.1016/S0021-9258(19)69940-0