Long-term maintenance of "cloned" human PLT cells in TCGF with LCL cells as a feeder layer

Long-term maintenance of "cloned" human PLT cells in TCGF with LCL cells as a feeder layer

The long‐term maintenance of T cells “cloned” by limiting dilution in TCGF was enhanced by the use of irradiated autologous lymphoblastoid cell line (LCL) cells as well as irradiated LCL cells of the individual to which the T cells were originally primed. It was possible to obtain more than 1 × 1012...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:J. Supramol. Struct.; (United States) 1980, Vol.13 (4), p.525-532
Hauptverfasser: Hank, Jacquelyn A., Inouye, Hiroo, Guy, Lynn A., Alter, Barbara J., Bach, Fritz H.
Format: Artikel
Sprache:eng
Schlagworte:
550300 - Cytology
BASIC BIOLOGICAL SCIENCES
CELL CULTURES
Cell Division - drug effects
Cell Line
Cells, Cultured
CLONE CELLS
CLONING
CULTURE MEDIA
HLA-D
Humans
Interleukin-2 - pharmacology
Lymphocyte Activation - drug effects
Lymphokines - pharmacology
Methods
PLT lymphocyte typing
T lymphocytes
T-Lymphocytes - physiology
T-Lymphocytes - radiation effects
TCGF
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The long‐term maintenance of T cells “cloned” by limiting dilution in TCGF was enhanced by the use of irradiated autologous lymphoblastoid cell line (LCL) cells as well as irradiated LCL cells of the individual to which the T cells were originally primed. It was possible to obtain more than 1 × 1012 cells from a “clone” seeded at one cell per well. Some of the clones tested express primed LD‐typing activity.
ISSN:0091-7419
1547-9366
DOI:10.1002/jss.400130411