ANTIBODIES AGAINST BACTERIAL AND VIRAL AGENTS IN SWINE FROM FAMILY-RUN FARMS IN MONTE NEGRO COUNTY, STATE OF RONDONIA, BRAZIL

Sera from swine from family-employed farms in Monte Negro County, state of Rondonia, Brazil, were evaluated against bacterial and viral agents. Brucellosis was evaluated by Rose-Bengal agglutination (RBA), standard tube agglutination (STA), and mercaptoethanol (ME); and leptospirosis by microscopic agglutination test (MAT) against 24 leptospira serovars. Anti-porcine parvovirus antibodies were tested by haemmaglutination inhibition assay, and ELISA technique was used for detection of antibodies against classical swine fever virus and Aujeszky disease virus. Only 1 (0.9%) from 104 tested sera reacted by RBA, and no samples reacted in STA and ME. Leptospira spp. antibodies were detected in 29 (32.9%) of 88 tested sera and the most frequent serovars detected were Castellonis, Bratislava and Canicola. Antibodies against porcine parvovirus were detected in 7.7% (8/104) of the sera. None of the examined samples reacted to classical swine fever virus or Aujeszky disease virus.Original Abstract: Foram avaliados soros de suinos provenientes de propriedades rurais que desenvolvem agricultura familiar no Municipio de Monte Negro, Rondonia, frente a agentes bacterianos e virais. Para a pesquisa de anticorpos contra Brucella spp., foram utilizados o Antigeno Acidificado Tamponado (AAT), a Soroaglutinacao Lenta em Tubos (SAL) e a prova do 2-Mercaptoetanol (2-ME). Para a pesquisa de Leptospira spp. utilizou-se a Soroaglutinacao Microscopica (SAM) contra 24 sorovares de leptospira. Anticorpos contra Parvovirus Suino (PVS) foram pesquisados pela Inibicao da Hemaglutinacao (IH), enquanto que o virus da Peste Suina Classica (PSC) e da Doenca de Aukeszky (DA) por Ensaio Imunoenzimatico (ELISA). Apenas uma (0,9%) das 104 amostras testadas foi reagente na AAT e nenhuma na SAL e 2-ME. Para Leptospira spp. foram testados 88 soros, sendo detectados anticorpos em 29 amostras (32,9%) e os sorovares mais frequentes foram Castellonis, Bratislava e Canicola. Foram detectados anticorpos contra PVS em 7,7% (8/104) dos soros testados. Nenhuma amostra foi reagente para PSC e DA.