Preparation of membrane vesicles in lithium chloride from cells of Nitrosomonas europaea

Preparation of membrane vesicles in lithium chloride from cells of Nitrosomonas europaea

The preparation of membrane vesicles in lithium chloride is described which oxidizes NH 4Cl via hydroxylamine to nitrite. The following properties of the vesicles were determined: the uptake of NH 4Cl and O 2 by electrode methods, the production of NH 2OH and NO 2 from NH 4Cl as well as ATPase activ...

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Veröffentlicht in:Analytical biochemistry 1980-01, Vol.109 (2), p.330-337
Hauptverfasser: Bhandari, Basant, Nicholas, D.J.D.
Format: Artikel
Sprache:eng
Schlagworte:
Adenosine Triphosphatases - analysis
Cell Fractionation - methods
Cell Membrane - metabolism
Cell Membrane - ultrastructure
Chlorides
Hydroxylamines - analysis
Lithium
Lithium Chloride
Microscopy, Electron
Nitrites - analysis
Nitrosomonas - metabolism
Nitrosomonas - ultrastructure
Oxygen Consumption
Spheroplasts - metabolism
Spheroplasts - ultrastructure
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Zusammenfassung:The preparation of membrane vesicles in lithium chloride is described which oxidizes NH 4Cl via hydroxylamine to nitrite. The following properties of the vesicles were determined: the uptake of NH 4Cl and O 2 by electrode methods, the production of NH 2OH and NO 2 from NH 4Cl as well as ATPase activity. The stoichiometry of NH 4 +:O 2:NO 2 − was found to be 1:1.2:0.7 in vesicles compared with 1:1.5:1.0 in either spheroplasts or washed cells. It is shown that the membrane vesicles also contain a Cu and energy-dependent NH 4 − translocase as in spheroplasts and cells.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(80)90656-9