Resonance assignment ofC-N-labeled snake neurotoxin II fromNaja oxiana

Neurotoxin II fromNaja oxiana venom is a short-chain snake curaremimetic neurotoxin containing four disulfide bonds. We obtainedC-N-labeled neurotoxin II to study its internal dynamics and surface properties with atomic resolution. The recombinant protein has the native spatial structure and is biol...

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Veröffentlicht in:	Applied magnetic resonance 2003-06, Vol.24 (2), p.247-254
Hauptverfasser:	Bocharov, E V, Lyukmanova, E N, Ermolyuk, Y S, Schulga, A A, Pluzhnikov, K A, Dolgikh, D A, Kirpichnikov, M P, Arseniev, A S
Format:	Artikel
Sprache:	eng
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Zusammenfassung:	Neurotoxin II fromNaja oxiana venom is a short-chain snake curaremimetic neurotoxin containing four disulfide bonds. We obtainedC-N-labeled neurotoxin II to study its internal dynamics and surface properties with atomic resolution. The recombinant protein has the native spatial structure and is biologically active. The nearly complete assignment ofH,C andN resonances for neurotoxin II was obtained by heteronuclear triple-resonance nuclear magnetic resonance spectroscopy. Analysis of the secondary chemical shifts of theH,C,C andCO nuclei reveal their strong correlation with the protein secondary structure.
ISSN:	0937-9347 1613-7507
DOI:	10.1007/BF03166664