Isolation and characterization of site-specific DNA-methyltransferases from Bacillus coagulans K

Two site-specific DNA methyltransferases, M.BcoKIA and M.BcoKIB, were isolated from the thermophilic strain Bacillus coagulans K. Each of the methylases protects the recognition site 5'-CTCTTC-3'/5'-GAAGAG-3' from cleavage with the cognate restriction endonuclease BcoKI. It is sh...

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Veröffentlicht in:	Biochemistry (Moscow) 2004-03, Vol.69 (3), p.299-305
Hauptverfasser:	Svadbina, I V, Zelinskaya, N V, Kovalevskaya, N P, Zheleznaya, L A, Matvienko, N I
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine Adenine - chemistry Bacillus - enzymology Bacillus coagulans Chromatography, Liquid Cytosine Cytosine - chemistry Deoxyribonucleases, Type II Site-Specific - chemistry Deoxyribonucleic acid DNA DNA Methylation DNA Modification Methylases - chemistry DNA Modification Methylases - isolation & purification DNA-Cytosine Methylases - chemistry DNA-Cytosine Methylases - isolation & purification Methyltransferases Microbiology Nucleases Site-Specific DNA-Methyltransferase (Adenine-Specific) - chemistry Site-Specific DNA-Methyltransferase (Adenine-Specific) - isolation & purification Substrate Specificity
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Zusammenfassung:	Two site-specific DNA methyltransferases, M.BcoKIA and M.BcoKIB, were isolated from the thermophilic strain Bacillus coagulans K. Each of the methylases protects the recognition site 5'-CTCTTC-3'/5'-GAAGAG-3' from cleavage with the cognate restriction endonuclease BcoKI. It is shown that M.BcoKIB is an N6-adenine specific methylase and M.BcoKIA is an N4-cytosine specific methylase. According to bisulfite mapping, M.BcoKIA methylates the first cytosine in the sequence 5'-CTCTTC-3'.
ISSN:	0006-2979 1608-3040
DOI:	10.1023/b:biry.0000022061.29918.8b