Novel chitosan/gold-MPA nanocomposite for sequence-specific oligonucleotide detection
A 20-mer single-stranded oligodeoxyribonucleotide (ssODNs) was covalently probed onto the nanocomposite electrode, made up of an indium-tin oxide (ITO) glass surface coated with chitosan (CHIT), which is bonded with carboxyl functionalized thiol capped gold nanoparticles (gold-mercaptopropionic acid...
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Veröffentlicht in: | Carbohydrate polymers 2010-08, Vol.82 (1), p.189-194 |
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Hauptverfasser: | , , , , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A 20-mer single-stranded oligodeoxyribonucleotide (ssODNs) was covalently probed onto the nanocomposite electrode, made up of an indium-tin oxide (ITO) glass surface coated with chitosan (CHIT), which is bonded with carboxyl functionalized thiol capped gold nanoparticles (gold-mercaptopropionic acid, Au-MPA NPs). The prepared CHIT/Au-MPA/ITO and the ssODNs/CHIT/Au-MPA/ITO electrodes were characterized with FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and cyclic voltammetry (CV). TEM micrograph of the composite electrode showed that Au-MPA NPs had the diameter ranging from 4 to 16
nm. The ssODNs/CHIT/Au-MPA/ITO electrode showed a linear increase in the amperometric current with an increasing concentration of the single-stranded target complementary ODNs (cODNs) within the range of 0.03–325
fM. The ssODNs/CHIT/Au-MPA/ITO electrode exhibited a sensitivity of 0.572
μA/fM with a response time of 12
s. The ssODNs/CHIT/Au-MPA/ITO electrode had a shelf life of ∼182 days, when stored at 4
°C. |
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ISSN: | 0144-8617 1879-1344 |
DOI: | 10.1016/j.carbpol.2010.04.051 |