Restriction Endonuclease Cleavage Maps of the Ampicillin Transposons Tn2601 and Tn2602

This paper reports the cleavage maps of ampicillin transposons Tn2601 and Tn2602, for restriction endonucleases BamHI, PvuII, AvaI, HincII, and HaeII. Both of the transposons are very similar to the well-known ampicillin transposon Tn3 in size, endonuclease cleavage sites, and possession of a short...

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Veröffentlicht in:MICROBIOLOGY and IMMUNOLOGY 1980/12/20, Vol.24(12), pp.1139-1149
Hauptverfasser: Yamamoto, Tomoko, Yamagata, Somay, Hashimoto, Yohko, Yamagishi, Saburo
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Sprache:eng
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Zusammenfassung:This paper reports the cleavage maps of ampicillin transposons Tn2601 and Tn2602, for restriction endonucleases BamHI, PvuII, AvaI, HincII, and HaeII. Both of the transposons are very similar to the well-known ampicillin transposon Tn3 in size, endonuclease cleavage sites, and possession of a short inverted repeat sequence at both ends. A slight difference in the cleavage pattern among these three transposons was observed in the region around the BamHI site which was assumed to be a part of the repressor gene for transposition.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1980.tb02919.x