Dual fluorescent protein reporters for studying cell behaviors in vivo
HeLa cells transfected with plasmids expressing dual fluorescent protein reporters with cherry, green, and cyan fluorscent proteins. Histone 2B fusions localize fluorescent proteins to the nucleus, while fluorescent proteins fused to glycosylphosphatidylinositol anchor signal sequence localizes to p...
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Veröffentlicht in: | Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2009-10, Vol.47 (10), p.spcone-spcone |
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creator | Stewart, M. David Jang, Chuan-Wei Hong, Nicholas W. Austin, Anthony P. Behringer, Richard R. |
description | HeLa cells transfected with plasmids expressing dual fluorescent protein reporters with cherry, green, and cyan fluorscent proteins. Histone 2B fusions localize fluorescent proteins to the nucleus, while fluorescent proteins fused to glycosylphosphatidylinositol anchor signal sequence localizes to plasma membranes. These dual fluorescent reporters label nuclear and plasma membranes of live cells to follow their behaviors in developing embryos. See the paper by Stewart et al. in this issue. |
doi_str_mv | 10.1002/dvg.20582 |
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title | Dual fluorescent protein reporters for studying cell behaviors in vivo |
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