Dual fluorescent protein reporters for studying cell behaviors in vivo

Dual fluorescent protein reporters for studying cell behaviors in vivo

HeLa cells transfected with plasmids expressing dual fluorescent protein reporters with cherry, green, and cyan fluorscent proteins. Histone 2B fusions localize fluorescent proteins to the nucleus, while fluorescent proteins fused to glycosylphosphatidylinositol anchor signal sequence localizes to p...

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Veröffentlicht in:Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2009-10, Vol.47 (10), p.spcone-spcone
Hauptverfasser: Stewart, M. David, Jang, Chuan-Wei, Hong, Nicholas W., Austin, Anthony P., Behringer, Richard R.
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Sprache:eng
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Zusammenfassung:HeLa cells transfected with plasmids expressing dual fluorescent protein reporters with cherry, green, and cyan fluorscent proteins. Histone 2B fusions localize fluorescent proteins to the nucleus, while fluorescent proteins fused to glycosylphosphatidylinositol anchor signal sequence localizes to plasma membranes. These dual fluorescent reporters label nuclear and plasma membranes of live cells to follow their behaviors in developing embryos. See the paper by Stewart et al. in this issue.
ISSN:1526-954X
1526-968X
DOI:10.1002/dvg.20582