Structure of the Oligosaccharide Chain of the SR-Type Lipopolysaccharide of Ralstonia solanacearum Toudk-2

Aiming at improving classification and taxonomy of Gram-negative phytopathogenic bacteria, we studied the structure of the lipopolysaccharide of Ralstonia solanacearum. Mild acid hydrolysis of the lipopolysaccharide of strain Toudk-2 followed by gel chromatography resulted in an O-polysaccharide and...

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Veröffentlicht in:Biomacromolecules 2008-08, Vol.9 (8), p.2215-2220
Hauptverfasser: Zdorovenko, Evelina L, Vinogradov, Evgeny, Wydra, Kerstin, Lindner, Buko, Knirel, Yuriy A
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Sprache:eng
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Zusammenfassung:Aiming at improving classification and taxonomy of Gram-negative phytopathogenic bacteria, we studied the structure of the lipopolysaccharide of Ralstonia solanacearum. Mild acid hydrolysis of the lipopolysaccharide of strain Toudk-2 followed by gel chromatography resulted in an O-polysaccharide and two oligosaccharide fractions. The smallest-size oligosaccharide fraction was studied by sugar analysis, high-resolution electrospray ionization mass spectrometry, and, after fractionation by anion-exchange chromatography on HiTrap Q, by one- and two-dimensional 1H and 13C NMR spectroscopy. It was found that the isolated oligosaccharides consist of the lipopolysaccharide core with one O-polysaccharide repeat (O-unit) attached. The core exists in two major glycoforms differing from each other in a lateral octulosonic acid residue, which is either d-glycero-d-talo-oct-2-ulosonic acid or 3-deoxy-d-manno-oct-2-ulosonic acid. A peculiar feature of the core is the occurrence of 4-amino-4-deoxy-l-arabinose nonstoichiometrically linked to a heptose residue. The full structures of the core and the biological O-unit as well as the site of the attachment of the O-unit to the core were established.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm800326u