Evidence for a Bicarbonate 'Escort' Site in Haemophilus influenzae b-Carbonic Anhydrase

Evidence for a Bicarbonate 'Escort' Site in Haemophilus influenzae b-Carbonic Anhydrase

The Haemophilus influenzae *b-carbonic anhydrase (HICA) allosteric site variants V47A and G41A were overexpressed and purified to homogeneity. These variants have kcat/Km values similar to that of the wild-type enzyme and exhibit a similar dramatic decrease in catalytic activity at pH

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Veröffentlicht in:Biochemistry (Easton) 2010-04, Vol.49 (17), p.3640-3647
Hauptverfasser: Rowlett, Roger S, Hoffmann, Katherine M, Failing, Hannah, Mysliwiec, Margaret M, Samardzic, Dejan
Format: Artikel
Sprache:eng
Schlagworte:
Haemophilus influenzae
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Zusammenfassung:The Haemophilus influenzae *b-carbonic anhydrase (HICA) allosteric site variants V47A and G41A were overexpressed and purified to homogeneity. These variants have kcat/Km values similar to that of the wild-type enzyme and exhibit a similar dramatic decrease in catalytic activity at pH
ISSN:0006-2960
DOI:10.1021/bi100328j