Single-Step DNA Immobilization on Antifouling Self-Assembled Monolayers Covalently Bound to Silicon (111)

Hydrosilylation of alkenes with epoxide-terminated tri(ethylene oxide) moieties on Si−H surfaces yields homogeneous monolayers for the efficient coupling of biomolecules. The wetting properties of the epoxide-functionalized surface allow for the spotting of solutions of biomolecules, making the surface amenable to microarraying. Immobilization of thiolated DNA was achieved in a single step to fabricate biorecognition interfaces showing the hybridization of complementary DNA at low concentrations and negligible binding of noncomplementary DNA.