The water cycle in closed ecological systems: Perspectives from the Biosphere 2 and Laboratory Biosphere systems

To achieve sustainable, healthy closed ecological systems requires solutions to challenges of closing the water cycle – recycling wastewater/irrigation water/soil medium leachate and evaporated water and supplying water of required quality as needed for different needs within the facility. Engineering Biosphere 2, the first multi-biome closed ecological system within a total airtight footprint of 12,700 m 2 with a combined volume of 200,000 m 3 with a total water capacity of some 6 × 10 6 L of water was especially challenging because it included human inhabitants, their agricultural and technical systems, as well as five analogue ecosystems ranging from rainforest to desert, freshwater ecologies to saltwater systems like mangrove and mini-ocean coral reef ecosystems. By contrast, the Laboratory Biosphere – a small (40 m 3 volume) soil-based plant growth facility with a footprint of 15 m 2 – is a very simplified system, but with similar challenges re salinity management and provision of water quality suitable for plant growth. In Biosphere 2, water needs included supplying potable water for people and domestic animals, irrigation water for a wide variety of food crops, and recycling and recovering soil nutrients from wastewater. In the wilderness biomes, providing adequately low salinity freshwater terrestrial ecosystems and maintaining appropriate salinity and pH in aquatic/marine ecosystems were challenges. The largest reservoirs in Biosphere 2 were the ocean/marsh with some 4 × 10 6 L, soil with 1 to 2 × 10 6 l, primary storage tank with 0 to 8 × 10 5 L and storage tanks for condensate and soil leachate collection and mixing tanks with a capacity of 1.6 × 10 5 L to supply irrigation for farm and wilderness ecosystems. Other reservoirs were far smaller – humidity in the atmosphere (2 × 10 3 L), streams in the rainforest and savannah, and seasonal pools in the desert were orders of magnitude smaller (8 × 10 4 L). Key technologies included condensation from humidity in the air handlers and from the glass space frame to produce high quality freshwater, wastewater treatment with constructed wetlands and desalination through reverse osmosis and flash evaporation were key to recycling water with appropriate quality throughout the Biosphere 2 facility. Wastewater from all human uses and the domestic animals in Biosphere 2 was treated and recycled through a series of constructed wetlands, which had hydraulic loading of 0.9–1.1 m 3 day −1 (240–290 gal d −1). Plant