THE HISTOCHEMISTRY OF THE TESTIS TISSUES FROM PATIENTS OF IDIOPATHIC MALE INFERTILITY: Report II. Studies on Complex Carbohydrate (Localization of Galactose Residues)

In the testis tissues from patients of idiopathic male infertility, complex carbohydrates have been histochemically studied by the light microscopic method, in comparison with the substances in tissues from normal individuals. In the present work, Peroxidase labeled-Ricinus communis agglutinin-diaminobezidine (PO-RCA-DAB), Concanavalin A-peroxidase-diaminobenzidine (Con A-PO-DAB), Con A-PO-DAB-periodic acid-m-aminophenol Fast black salt K (PA-AP-FBK) reactions and protein reactions are employed histochemically. In the infertile testis tissues and normal tesis tissues, the seminiferous tubules and interstitium contain an appreciable amount of α-D-glucose and α-D-mannose. In the seminiferous tubulus and interstitium of the idiopathic male infertile testis tissues, another histochemical property of primary importance was the galactose deficiency in complex carbohydrates involved, which are in nature a glycoprotein containing only small amounts of sulhydryl and disulphide groups. The results of the present study indicate that the PO-RCA-DAB procedure is useful in diagnosing the idiopathic male infertility.