Quantitative Estimation of Secretory IgA in Inflamed Human Gingival Tissue

The purpose of this study was to determine the secretory IgA (S-IgA) in human serum and in gingival tissue and to compare their concentrations with clinical findings. The subjects consisted of 8 male and 15 female patients. Some of specimes were stained to observe the location of S-IgA by fluorescein antibody method. Before periodontal surgery, the serum was obtained and clinical fndings such as Gingival Index, pocket depth and Plaque Index were recorded. The gingival tissues were obtained during periodontal surgery. To extract S-IgA, the gingival tissue was eluted by phosphate buffered saline (PBS) and glycine hydrochloride buffer (GHB). The results were as follows: 1. The concentration of S-IgA in human serum was 0.213mg/dl. 2. The concentration of S-IgA in gingival tissue extracted by PBS was 31.4μg/g Dry Gingival Weight. 3. The concentration of S-IgA in gingival tissue obtained by GHB was 4.98μg/g Dry Gingival Weight. 4. The S-IgA/albumine ratio in gingival extract by PBS was higher than that in serum (p