The Relationship of Urine Acid Mucopolysaccharide and Urine Total Binding Hexose in Fractions by Gel Filtration Column Chromatography to the Age and Glucose Tolerance

Sephadex G-15 column chromatography on Acid Mucopolysaccharide (AMPS) and Total Binding Hexose (TBH) in 24-hour urine specimens were performed to clarify the relationship between these substances and age as well as glucose tolerance. The examinees were 5 cases of young non-diabetics from 20 to 30 years of age, 5 cases of aged non-diabetics over 60 years old and 6 cases of aged diabetics over 60 years old. In this study, the diabetics were treated with sulfonylureas, but poorly controlled. AMPS and TBH in 24-hour urine specimens were fractionated into three fractions of Fr-I, Fr-II and Fr-III by sephadex G-15 column chromatography. Molecular weight is in order of Fr-I>Fr-II>Fr-III. 1) In non-diabetics, the amount of 24-hour urine AMPS is lower in the aged than that in the young. Also, each amount of AMPS in three fractions is lower in the aged than that in the young. 2) The amount of 24-hour urine AMPS tends to be higher in the aged diabetics than that in the aged non-diabetics. In each AMPS of Fr-I and Fr-II, there is no difference between aged non-diabetics and aged diabetics. Only the AMPS of Fr-III is higher in the aged diabetics than that in the aged non-diabetics. Accordingly, the higher level of 24-hour urine AMPS in the aged diabetics is caused by the higher level of Fr-III AMPS. 3) In non-diabetics, the amount of 24-hour urine TBH is lower in the aged than that in the young. Also, each amount of TBH in three fractions is lower or tends to be lower in the aged than that in the young. 4) The amount of 24-hour urine TBH is higher in the aged diabetics than that in the aged non-diabetics. Also, each amount of TBH in three fractions is lower or tends to be lower in the aged non-diabetics than that in the aged diabetics. Age and glucose intolerance are accelerating factors for arteriosclerosis. But the present results may suggest that these factors have different roles on the progressive course of arteriosclerosis.