A sensitive fluorimetric rate assay of arylsulfatases

A sensitive fluorimetric rate assay of arylsulfatases

A fluorimetric rate assay for arylsulfatases is described which utilizes the special fluorescence spectral properties of 2-naphthol, the hydrolysis product of the substrate employed. The technique is about 50-fold more sensitive than absorption spectral methods and can be used over a wide pH range.

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Veröffentlicht in:Analytical biochemistry 1980-11, Vol.108 (2), p.402-405
Hauptverfasser: Lynn, K.R., Szabo, A.G., Krajcarski, D., Chuaqui, C.A.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Arylsulfatases - analysis
Helix (Snails) - enzymology
Hydrolysis
Spectrometry, Fluorescence
Sulfatases - analysis
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Zusammenfassung:A fluorimetric rate assay for arylsulfatases is described which utilizes the special fluorescence spectral properties of 2-naphthol, the hydrolysis product of the substrate employed. The technique is about 50-fold more sensitive than absorption spectral methods and can be used over a wide pH range.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(80)90605-3