A new enzymatic method for colorimetric determination of free fatty acids

A new enzymatic method for colorimetric determination of free fatty acids

A new enzymatic method using acyl-CoA synthetase, acyl-CoA oxidase, and peroxidase was established for the colorimetric determination of long-chain free fatty acids. The procedure consists of only two steps: the synthesis of acyl-CoA from the free fatty acids and the oxidation of acyl-CoA in the pre...

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Veröffentlicht in:Analytical biochemistry 1980-10, Vol.108 (1), p.6-10
Hauptverfasser: Mizuno, Koji, Toyosato, Mitsuyoshi, Yabumoto, Shinichi, Tanimizu, Isao, Hirakawa, Hakudo
Format: Artikel
Sprache:eng
Schlagworte:
Candida - enzymology
Coenzyme A Ligases
Colorimetry - methods
Fatty Acid Desaturases
Fatty Acids, Nonesterified - analysis
Fatty Acids, Nonesterified - blood
Humans
Kinetics
Oxygen Consumption
Peroxidases
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Zusammenfassung:A new enzymatic method using acyl-CoA synthetase, acyl-CoA oxidase, and peroxidase was established for the colorimetric determination of long-chain free fatty acids. The procedure consists of only two steps: the synthesis of acyl-CoA from the free fatty acids and the oxidation of acyl-CoA in the presence of N-ethylmaleimide at 37°C. Compared to conventional methods, this method has a high specificity for free fatty acids, is reproducible, is applicable to smaller quantities of sample, and can be completed within a shorter period of time.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(80)90686-7