Microarray-Assisted High-Throughput Identification of a Cell-Permeable Small-Molecule Binder of 14-3-3 Proteins

The way to a 14‐3‐3 binder: A fragment‐based combinatorial small‐molecule microarray generates affinity‐based fingerprints of the 14‐3‐3σ protein. One small molecule (see picture; in red box) that disrupts the 14‐3‐3/protein interaction (green/blue) has been identified. The compound is cell‐permeabl...

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Veröffentlicht in:	Angewandte Chemie (International ed.) 2010-09, Vol.49 (37), p.6528-6532
Hauptverfasser:	Wu, Hao, Ge, Jingyan, Yao, Shao Q
Format:	Artikel
Sprache:	eng
Schlagworte:	14-3-3 Proteins - genetics 14-3-3 Proteins - metabolism Animals Cell Line combinatorial chemistry fragment-based screening High-Throughput Screening Assays - methods Humans microarrays Molecular Structure Protein Array Analysis - methods Protein Isoforms - metabolism protein-protein interactions proteins
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Zusammenfassung:	The way to a 14‐3‐3 binder: A fragment‐based combinatorial small‐molecule microarray generates affinity‐based fingerprints of the 14‐3‐3σ protein. One small molecule (see picture; in red box) that disrupts the 14‐3‐3/protein interaction (green/blue) has been identified. The compound is cell‐permeable and possesses both in vitro and in‐cell activities.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201003257