Polymeric micelle gene delivery of bcl-XL via eye drop reduced corneal apoptosis following epithelial debridement
Stromal keratocyte apoptosis triggered by epithelial injury is one mechanism of corneal disorders. A model of epithelial injury by epithelial debridement is established, and keratocyte apoptosis is evidenced by DNA fragmentation and cellular morphological changes in the anterior stroma underlying th...
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| Veröffentlicht in: | Journal of controlled release 2010-10, Vol.147 (1), p.76-83 |
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| creator | TONG, Yaw-Chong CHANG, Shwu-Fen KAO, Winston W.-Y LIU, Chia-Yang LIAW, Jiahorng |
| description | Stromal keratocyte apoptosis triggered by epithelial injury is one mechanism of corneal disorders. A model of epithelial injury by epithelial debridement is established, and keratocyte apoptosis is evidenced by DNA fragmentation and cellular morphological changes in the anterior stroma underlying the injured epithelium. Delivery of plasmid (pCMV-bcl-x(L)-eGFP) encoding an anti-apoptotic gene, the bcl-x(L) with a nano-carrier, polymeric micelles (PM) via eye drop to cornea after epithelial debridement, the mRNA level of bcl-x(L) was significantly increased (2.2-fold, P |
| doi_str_mv | 10.1016/j.jconrel.2010.06.006 |
| format | Article |
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A model of epithelial injury by epithelial debridement is established, and keratocyte apoptosis is evidenced by DNA fragmentation and cellular morphological changes in the anterior stroma underlying the injured epithelium. Delivery of plasmid (pCMV-bcl-x(L)-eGFP) encoding an anti-apoptotic gene, the bcl-x(L) with a nano-carrier, polymeric micelles (PM) via eye drop to cornea after epithelial debridement, the mRNA level of bcl-x(L) was significantly increased (2.2-fold, P<0.05) at 48 h and the eGFP mRNA was detected (4571.7 ± 1194.5 copies/μg total RNA). The bcl-x(L)-eGFP fusion protein was also detected in wounded cornea at 48 h after delivery, accompanying with decreased DNA fragmentation and lower caspase-3 activity (P<0.05). In conclusion, eye drop of pCMV-bcl-x(L)-eGFP/PM reduced corneal apoptosis following epithelial debridement.</description><identifier>ISSN: 0168-3659</identifier><identifier>EISSN: 1873-4995</identifier><identifier>DOI: 10.1016/j.jconrel.2010.06.006</identifier><identifier>PMID: 20600407</identifier><identifier>CODEN: JCREEC</identifier><language>eng</language><publisher>Kidlington: Elsevier</publisher><subject>Animals ; Apoptosis ; bcl-X Protein - biosynthesis ; bcl-X Protein - genetics ; Biological and medical sciences ; Corneal Diseases - therapy ; Cytomegalovirus - genetics ; Drug Carriers - chemistry ; Epithelium, Corneal - injuries ; Epithelium, Corneal - metabolism ; Epithelium, Corneal - pathology ; Gene Transfer Techniques ; General pharmacology ; Genetic Vectors - chemistry ; Green Fluorescent Proteins - genetics ; In Situ Nick-End Labeling ; Male ; Medical sciences ; Mice ; Mice, Nude ; Micelles ; Microscopy, Electron, Transmission ; Microscopy, Fluorescence ; Ophthalmic Solutions ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Plasmids ; Polyethylene Glycols - chemistry ; Promoter Regions, Genetic ; Propylene Glycols - chemistry ; Recombinant Fusion Proteins - biosynthesis ; Recombinant Fusion Proteins - genetics</subject><ispartof>Journal of controlled release, 2010-10, Vol.147 (1), p.76-83</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c253t-98c7bdb54de3b0ee59665661905c4d04b3091fa97033aae854e8c801b0df378e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23288007$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20600407$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TONG, Yaw-Chong</creatorcontrib><creatorcontrib>CHANG, Shwu-Fen</creatorcontrib><creatorcontrib>KAO, Winston W.-Y</creatorcontrib><creatorcontrib>LIU, Chia-Yang</creatorcontrib><creatorcontrib>LIAW, Jiahorng</creatorcontrib><title>Polymeric micelle gene delivery of bcl-XL via eye drop reduced corneal apoptosis following epithelial debridement</title><title>Journal of controlled release</title><addtitle>J Control Release</addtitle><description>Stromal keratocyte apoptosis triggered by epithelial injury is one mechanism of corneal disorders. A model of epithelial injury by epithelial debridement is established, and keratocyte apoptosis is evidenced by DNA fragmentation and cellular morphological changes in the anterior stroma underlying the injured epithelium. Delivery of plasmid (pCMV-bcl-x(L)-eGFP) encoding an anti-apoptotic gene, the bcl-x(L) with a nano-carrier, polymeric micelles (PM) via eye drop to cornea after epithelial debridement, the mRNA level of bcl-x(L) was significantly increased (2.2-fold, P<0.05) at 48 h and the eGFP mRNA was detected (4571.7 ± 1194.5 copies/μg total RNA). The bcl-x(L)-eGFP fusion protein was also detected in wounded cornea at 48 h after delivery, accompanying with decreased DNA fragmentation and lower caspase-3 activity (P<0.05). In conclusion, eye drop of pCMV-bcl-x(L)-eGFP/PM reduced corneal apoptosis following epithelial debridement.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>bcl-X Protein - biosynthesis</subject><subject>bcl-X Protein - genetics</subject><subject>Biological and medical sciences</subject><subject>Corneal Diseases - therapy</subject><subject>Cytomegalovirus - genetics</subject><subject>Drug Carriers - chemistry</subject><subject>Epithelium, Corneal - injuries</subject><subject>Epithelium, Corneal - metabolism</subject><subject>Epithelium, Corneal - pathology</subject><subject>Gene Transfer Techniques</subject><subject>General pharmacology</subject><subject>Genetic Vectors - chemistry</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>In Situ Nick-End Labeling</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Micelles</subject><subject>Microscopy, Electron, Transmission</subject><subject>Microscopy, Fluorescence</subject><subject>Ophthalmic Solutions</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Plasmids</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Promoter Regions, Genetic</subject><subject>Propylene Glycols - chemistry</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Recombinant Fusion Proteins - genetics</subject><issn>0168-3659</issn><issn>1873-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1rGzEQhkVpaFy3P6FBl9LTOiNrpdUeQ0g_wNAcEshNaKVZV0a72kjrBP_7yMRJTwMzzzvDPIR8Y7BiwOTlbrWzcUwYVmsoPZArAPmBLJhqeFW3rfhIFoVTFZeiPSefc94BgOB184mcr0EC1NAsyONtDIcBk7d08BZDQLrFEanD4J8wHWjsaWdD9bChT95QPJRRihNN6PYWHbUxjWgCNVOc5ph9pn0MIT77cUtx8vO_sqeMHXbJOxxwnL-Qs96EjF9PdUnuf97cXf-uNn9__bm-2lR2Lfhctco2netE7ZB3gChaKYWUrAVhawd1x6FlvWkb4NwYVKJGZRWwDlzPG4V8SX687p1SfNxjnvXg8_FDM2LcZ92IuvgQTBRSvJI2xZwT9npKfjDpoBnoo2y90yfZ-ihbg9RFdsldnC7suwHde-rNbgG-nwCTrQl9MqP1-T_H10pB4V4APeuLvQ</recordid><startdate>201010</startdate><enddate>201010</enddate><creator>TONG, Yaw-Chong</creator><creator>CHANG, Shwu-Fen</creator><creator>KAO, Winston W.-Y</creator><creator>LIU, Chia-Yang</creator><creator>LIAW, Jiahorng</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201010</creationdate><title>Polymeric micelle gene delivery of bcl-XL via eye drop reduced corneal apoptosis following epithelial debridement</title><author>TONG, Yaw-Chong ; CHANG, Shwu-Fen ; KAO, Winston W.-Y ; LIU, Chia-Yang ; LIAW, Jiahorng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c253t-98c7bdb54de3b0ee59665661905c4d04b3091fa97033aae854e8c801b0df378e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>bcl-X Protein - biosynthesis</topic><topic>bcl-X Protein - genetics</topic><topic>Biological and medical sciences</topic><topic>Corneal Diseases - therapy</topic><topic>Cytomegalovirus - genetics</topic><topic>Drug Carriers - chemistry</topic><topic>Epithelium, Corneal - injuries</topic><topic>Epithelium, Corneal - metabolism</topic><topic>Epithelium, Corneal - pathology</topic><topic>Gene Transfer Techniques</topic><topic>General pharmacology</topic><topic>Genetic Vectors - chemistry</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>In Situ Nick-End Labeling</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Micelles</topic><topic>Microscopy, Electron, Transmission</topic><topic>Microscopy, Fluorescence</topic><topic>Ophthalmic Solutions</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Plasmids</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Promoter Regions, Genetic</topic><topic>Propylene Glycols - chemistry</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Recombinant Fusion Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TONG, Yaw-Chong</creatorcontrib><creatorcontrib>CHANG, Shwu-Fen</creatorcontrib><creatorcontrib>KAO, Winston W.-Y</creatorcontrib><creatorcontrib>LIU, Chia-Yang</creatorcontrib><creatorcontrib>LIAW, Jiahorng</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of controlled release</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TONG, Yaw-Chong</au><au>CHANG, Shwu-Fen</au><au>KAO, Winston W.-Y</au><au>LIU, Chia-Yang</au><au>LIAW, Jiahorng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polymeric micelle gene delivery of bcl-XL via eye drop reduced corneal apoptosis following epithelial debridement</atitle><jtitle>Journal of controlled release</jtitle><addtitle>J Control Release</addtitle><date>2010-10</date><risdate>2010</risdate><volume>147</volume><issue>1</issue><spage>76</spage><epage>83</epage><pages>76-83</pages><issn>0168-3659</issn><eissn>1873-4995</eissn><coden>JCREEC</coden><abstract>Stromal keratocyte apoptosis triggered by epithelial injury is one mechanism of corneal disorders. A model of epithelial injury by epithelial debridement is established, and keratocyte apoptosis is evidenced by DNA fragmentation and cellular morphological changes in the anterior stroma underlying the injured epithelium. Delivery of plasmid (pCMV-bcl-x(L)-eGFP) encoding an anti-apoptotic gene, the bcl-x(L) with a nano-carrier, polymeric micelles (PM) via eye drop to cornea after epithelial debridement, the mRNA level of bcl-x(L) was significantly increased (2.2-fold, P<0.05) at 48 h and the eGFP mRNA was detected (4571.7 ± 1194.5 copies/μg total RNA). The bcl-x(L)-eGFP fusion protein was also detected in wounded cornea at 48 h after delivery, accompanying with decreased DNA fragmentation and lower caspase-3 activity (P<0.05). In conclusion, eye drop of pCMV-bcl-x(L)-eGFP/PM reduced corneal apoptosis following epithelial debridement.</abstract><cop>Kidlington</cop><pub>Elsevier</pub><pmid>20600407</pmid><doi>10.1016/j.jconrel.2010.06.006</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Apoptosis bcl-X Protein - biosynthesis bcl-X Protein - genetics Biological and medical sciences Corneal Diseases - therapy Cytomegalovirus - genetics Drug Carriers - chemistry Epithelium, Corneal - injuries Epithelium, Corneal - metabolism Epithelium, Corneal - pathology Gene Transfer Techniques General pharmacology Genetic Vectors - chemistry Green Fluorescent Proteins - genetics In Situ Nick-End Labeling Male Medical sciences Mice Mice, Nude Micelles Microscopy, Electron, Transmission Microscopy, Fluorescence Ophthalmic Solutions Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Plasmids Polyethylene Glycols - chemistry Promoter Regions, Genetic Propylene Glycols - chemistry Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics |
| title | Polymeric micelle gene delivery of bcl-XL via eye drop reduced corneal apoptosis following epithelial debridement |
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