Polymeric micelle gene delivery of bcl-XL via eye drop reduced corneal apoptosis following epithelial debridement

Stromal keratocyte apoptosis triggered by epithelial injury is one mechanism of corneal disorders. A model of epithelial injury by epithelial debridement is established, and keratocyte apoptosis is evidenced by DNA fragmentation and cellular morphological changes in the anterior stroma underlying th...

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Veröffentlicht in:	Journal of controlled release 2010-10, Vol.147 (1), p.76-83
Hauptverfasser:	TONG, Yaw-Chong, CHANG, Shwu-Fen, KAO, Winston W.-Y, LIU, Chia-Yang, LIAW, Jiahorng
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis bcl-X Protein - biosynthesis bcl-X Protein - genetics Biological and medical sciences Corneal Diseases - therapy Cytomegalovirus - genetics Drug Carriers - chemistry Epithelium, Corneal - injuries Epithelium, Corneal - metabolism Epithelium, Corneal - pathology Gene Transfer Techniques General pharmacology Genetic Vectors - chemistry Green Fluorescent Proteins - genetics In Situ Nick-End Labeling Male Medical sciences Mice Mice, Nude Micelles Microscopy, Electron, Transmission Microscopy, Fluorescence Ophthalmic Solutions Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Plasmids Polyethylene Glycols - chemistry Promoter Regions, Genetic Propylene Glycols - chemistry Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics
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Zusammenfassung:	Stromal keratocyte apoptosis triggered by epithelial injury is one mechanism of corneal disorders. A model of epithelial injury by epithelial debridement is established, and keratocyte apoptosis is evidenced by DNA fragmentation and cellular morphological changes in the anterior stroma underlying the injured epithelium. Delivery of plasmid (pCMV-bcl-x(L)-eGFP) encoding an anti-apoptotic gene, the bcl-x(L) with a nano-carrier, polymeric micelles (PM) via eye drop to cornea after epithelial debridement, the mRNA level of bcl-x(L) was significantly increased (2.2-fold, P
ISSN:	0168-3659 1873-4995
DOI:	10.1016/j.jconrel.2010.06.006