A Cleavable Linker Based on the Levulinoyl Ester for Activity‐Based Protein Profiling

A Cleavable Linker Based on the Levulinoyl Ester for Activity‐Based Protein Profiling

Get linked: The title linker is stable under various biological conditions and can be cleaved chemoselectively with hydrazine (see scheme). Its use is demonstrated in the activity‐based enrichment and identification of proteasome active subunits from cell extracts.

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Bibliographische Detailangaben
Veröffentlicht in:Angewandte Chemie International Edition 2010-09, Vol.49 (38), p.6802-6805
Hauptverfasser: Geurink, Paul P., Florea, Bogdan I., Li, Nan, Witte, Martin D., Verasdonck, Joeri, Kuo, Chi‐Lin, van der Marel, Gijs A., Overkleeft, Herman S.
Format: Artikel
Sprache:eng
Schlagworte:
activity‐based profiling
Catalytic Domain
Cell Line
cleavable linkers
Humans
Hydrazines - chemistry
Levulinic Acids - chemistry
levulinoyl ester
Oligopeptides - chemistry
Proteasome Endopeptidase Complex - chemistry
proteasomes
Proteomics
Tandem Mass Spectrometry
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Beschreibung
Zusammenfassung:Get linked: The title linker is stable under various biological conditions and can be cleaved chemoselectively with hydrazine (see scheme). Its use is demonstrated in the activity‐based enrichment and identification of proteasome active subunits from cell extracts.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201001767