Dual-Color Imaging of Sodium/Calcium Ion Activities with Two-Photon Fluorescent Probes

Dual-Color Imaging of Sodium/Calcium Ion Activities with Two-Photon Fluorescent Probes

A two‐photon probe (BCaM) shows 14‐fold enhancement in two‐photon emission fluorescence in response to Ca2+ and shows high sensitivity and selectivity for near‐membrane Ca2+ ions (see picture). Combined with the known Na+ two‐photon probe ANa1, BCaM allows simultaneous dual‐color imaging of Ca2+/Na+...

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Veröffentlicht in:Angewandte Chemie (International ed.) 2010-09, Vol.49 (38), p.6786-6789
Hauptverfasser: Kim, Hyung Joong, Han, Ji Hee, Kim, Mi Kyung, Lim, Chang Su, Kim, Hwan Myung, Cho, Bong Rae
Format: Artikel
Sprache:eng
Schlagworte:
calcium
Calcium - chemistry
Color
fluorescence spectroscopy
Fluorescent Dyes - chemistry
HeLa Cells
Humans
imaging agents
live tissue
Microscopy, Fluorescence
Photons
Sodium - chemistry
two-photon microscopy
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Zusammenfassung:A two‐photon probe (BCaM) shows 14‐fold enhancement in two‐photon emission fluorescence in response to Ca2+ and shows high sensitivity and selectivity for near‐membrane Ca2+ ions (see picture). Combined with the known Na+ two‐photon probe ANa1, BCaM allows simultaneous dual‐color imaging of Ca2+/Na+ activities within live cells and in tissues at more than 100 μm depth for long time periods without photobleaching.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201002907