A chimeric vitronectin: IGF-I protein supports feeder-cell-free and serum-free culture of human embryonic stem cells

The therapeutic use of human embryonic stem (hES) cells is severely limited by safety concerns regarding their culture in media containing animal-derived or nondefined factors and on animal-derived feeder cells. Thus, there is a pressing need to develop culture techniques that are xeno-free, fully d...

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Veröffentlicht in:	Stem cells and development 2010-09, Vol.19 (9), p.1297-1305
Hauptverfasser:	Manton, Kerry J, Richards, Sean, Van Lonkhuyzen, Derek, Cormack, Luke, Leavesley, David, Upton, Zee
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell culture Cell Culture Techniques - methods Cell Differentiation - drug effects Cell Lineage - drug effects Cell Proliferation - drug effects Cells, Cultured Coculture Techniques Culture Media, Serum-Free - pharmacology Embryonic stem cells Embryonic Stem Cells - cytology Embryonic Stem Cells - drug effects Embryonic Stem Cells - metabolism Embryonic Stem Cells - physiology Evaluation Health aspects Humans Insulin-like growth factor 1 Insulin-Like Growth Factor I - chemistry Insulin-Like Growth Factor I - pharmacology Karyotyping Laminin Mice Physiological aspects Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - pharmacology Stem cell research Vitronectin - chemistry Vitronectin - pharmacology
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