Ultrastructural deformity of ovarian follicles induced by different cryopreservation protocols

Ultrastructural deformities were monitored after cryopreservation of F1 (B6CBAF1) mouse ovaries by either slow freezing or vitrification. Vacuole formation in the ooplasm, zona pellucida, and the cytoplasm of follicular cells, and mitochondrial deformity were detected. These types of cryodamage demo...

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Veröffentlicht in:Fertility and sterility 2010-09, Vol.94 (4), p.1548-1550.e1
Hauptverfasser: Kim, Gil Ah, M.S, Kim, Hwa Young, B.Sc, Kim, Jee Woong, M.S, Lee, Gene, D.D.S., Ph.D, Lee, Eunsong, D.V.M., Ph.D, Lim, Jeong Mook, D.V.M., Ph.D
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container_end_page 1550.e1
container_issue 4
container_start_page 1548
container_title Fertility and sterility
container_volume 94
creator Kim, Gil Ah, M.S
Kim, Hwa Young, B.Sc
Kim, Jee Woong, M.S
Lee, Gene, D.D.S., Ph.D
Lee, Eunsong, D.V.M., Ph.D
Lim, Jeong Mook, D.V.M., Ph.D
description Ultrastructural deformities were monitored after cryopreservation of F1 (B6CBAF1) mouse ovaries by either slow freezing or vitrification. Vacuole formation in the ooplasm, zona pellucida, and the cytoplasm of follicular cells, and mitochondrial deformity were detected. These types of cryodamage demonstrated protocol specificity.
doi_str_mv 10.1016/j.fertnstert.2009.12.029
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Obstetrics</subject><subject>Internal Medicine</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred CBA</subject><subject>Mouse ovary</subject><subject>Obstetrics and Gynecology</subject><subject>Ovarian Follicle - pathology</subject><subject>Ovarian Follicle - ultrastructure</subject><subject>Ovary</subject><subject>slow freezing</subject><subject>ultrastructure</subject><subject>vitrification</subject><subject>Zona Pellucida - pathology</subject><subject>Zona Pellucida - ultrastructure</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk2LFDEQQBtR3NnVvyC5iKceU8l0unMRdNFVWPCgezWk0xXImEnGJD3Q_940M7rgyUtSh1dfj2oaAnQLFMTb_dZiKiGX-m4ZpXILbEuZfNJsoOtE24mOP202lELXUjawq-Y65z2lVEDPnjdXjAJQOshN8-PBl6RzSbMpc9KeTGhjOriykGhJPOnkdCA2eu-Mx0xcmGaDExkXMjlbp8BQiElLPCbMmE66uBjIMcUSTfT5RfPMap_x5eW_aR4-ffx--7m9_3r35fb9fWu6figtFzgIRjXsau2RomWD5T0Iq9eYTZxJhkwDYyNIHCdmpey4tdIKCzsB_KZ5c65bO_-aMRd1cNmg9zpgnLPqOy5lL4BVcjiTJsWcE1p1TO6g06KAqlWu2qtHuWqVq4CpKremvro0mccDTn8T_9iswOsLoLPR3iYdjMuPHAcpB8Er9-HMYVVycphUNg5D3d0lNEVN0f3PNO_-KWK8C672_YkL5n2cU6jKFahcE9S39RjWW6CS7lgN-W_rsLUf</recordid><startdate>20100901</startdate><enddate>20100901</enddate><creator>Kim, Gil Ah, M.S</creator><creator>Kim, Hwa Young, B.Sc</creator><creator>Kim, Jee Woong, M.S</creator><creator>Lee, Gene, D.D.S., Ph.D</creator><creator>Lee, Eunsong, D.V.M., Ph.D</creator><creator>Lim, Jeong Mook, D.V.M., Ph.D</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100901</creationdate><title>Ultrastructural deformity of ovarian follicles induced by different cryopreservation protocols</title><author>Kim, Gil Ah, M.S ; Kim, Hwa Young, B.Sc ; Kim, Jee Woong, M.S ; Lee, Gene, D.D.S., Ph.D ; Lee, Eunsong, D.V.M., Ph.D ; Lim, Jeong Mook, D.V.M., Ph.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c578t-36e8620a14cedb0ef28f3716fa0ef22d3292e2a122b19ebd2f9953ff9f6f14613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>cryodamage</topic><topic>Cryopreservation - methods</topic><topic>Female</topic><topic>Freezing - adverse effects</topic><topic>Gynecology. 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source MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Animals
Biological and medical sciences
cryodamage
Cryopreservation - methods
Female
Freezing - adverse effects
Gynecology. Andrology. Obstetrics
Internal Medicine
Medical sciences
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Mouse ovary
Obstetrics and Gynecology
Ovarian Follicle - pathology
Ovarian Follicle - ultrastructure
Ovary
slow freezing
ultrastructure
vitrification
Zona Pellucida - pathology
Zona Pellucida - ultrastructure
title Ultrastructural deformity of ovarian follicles induced by different cryopreservation protocols
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