Ultrastructural deformity of ovarian follicles induced by different cryopreservation protocols

Ultrastructural deformities were monitored after cryopreservation of F1 (B6CBAF1) mouse ovaries by either slow freezing or vitrification. Vacuole formation in the ooplasm, zona pellucida, and the cytoplasm of follicular cells, and mitochondrial deformity were detected. These types of cryodamage demo...

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Veröffentlicht in:	Fertility and sterility 2010-09, Vol.94 (4), p.1548-1550.e1
Hauptverfasser:	Kim, Gil Ah, M.S, Kim, Hwa Young, B.Sc, Kim, Jee Woong, M.S, Lee, Gene, D.D.S., Ph.D, Lee, Eunsong, D.V.M., Ph.D, Lim, Jeong Mook, D.V.M., Ph.D
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences cryodamage Cryopreservation - methods Female Freezing - adverse effects Gynecology. Andrology. Obstetrics Internal Medicine Medical sciences Mice Mice, Inbred C57BL Mice, Inbred CBA Mouse ovary Obstetrics and Gynecology Ovarian Follicle - pathology Ovarian Follicle - ultrastructure Ovary slow freezing ultrastructure vitrification Zona Pellucida - pathology Zona Pellucida - ultrastructure
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Zusammenfassung:	Ultrastructural deformities were monitored after cryopreservation of F1 (B6CBAF1) mouse ovaries by either slow freezing or vitrification. Vacuole formation in the ooplasm, zona pellucida, and the cytoplasm of follicular cells, and mitochondrial deformity were detected. These types of cryodamage demonstrated protocol specificity.
ISSN:	0015-0282 1556-5653
DOI:	10.1016/j.fertnstert.2009.12.029