Binding of cytochalasin B to human erythrocyte glucose transporter

Cytochalasin B, a potent inhibitor of D-glucose transport systems, binds to the glucose transporter purified from human erythrocytes as described previously [Kasahara, M., & Hinkle, P. C. (1977) J. Biol. Chem. 252, 7384]. The transporter binds 9.2 +/- 1.3 nmol of cytochalasin B/mg of protein wit...

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Veröffentlicht in:Biochemistry (Easton) 1980-11, Vol.19 (23), p.5417-5420
Hauptverfasser: Sogin, David C, Hinkle, Peter C
Format: Artikel
Sprache:eng
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Zusammenfassung:Cytochalasin B, a potent inhibitor of D-glucose transport systems, binds to the glucose transporter purified from human erythrocytes as described previously [Kasahara, M., & Hinkle, P. C. (1977) J. Biol. Chem. 252, 7384]. The transporter binds 9.2 +/- 1.3 nmol of cytochalasin B/mg of protein with a dissociation constant of 0.18 microM. The binding is competitively inhibited by D-glucose (Ki = 43 mM). Phloretin, diethylstilbestrol, maltose, 6-O-propyl-D-galactose, propyl beta-D-glucopyranoside, and dithiothreitol were also linear competitive inhibitors of cytochalasin B binding. The propyl sugars have been shown to inhibit transport from either the plasma or cytoplasma side of the membrane, respectively. The binding of cytochalasin B to the isolated transporter was inhibited by both propyl sugars.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00564a041